Sophisticated Analytical Instrument Facility

CSIR-Central Drug Research Institute, Lucknow

Supported by: Department of Science & Technology ( Govt. of India)

SAIF Users are request to register on for online sample information submission and make online payment for analysis.

Mass spectrometry

Mass spectrometry is a powerful analytical technique that is used to determine the molecular weight and calculate molecular formula using high resolution mass spectrometry. The MS principle consists of ionizing chemical compounds to generate charged molecules or molecule fragments and measurement of their mass-to-charge ratios. The combination of liquid chromatography (LC), photodiode-array UV-Vis detector and atmospheric pressure ionization mass spectrometry (APIMS) has proven to be a useful on-line system for establishing the identity of components in given test samples. In recent era, the tandem mass spectrometry (MS/MS) is used to identify unknown compounds, to quantify known compounds, and to elucidate the structure and chemical properties of molecules.

SAIF-CDRI Lucknow has following facilities
  • Electrospray Ionization Mass Spectrometry
  • Atmospheric Pressure Chemical Ionization Mass Spectrometry
  • Ultra-High Performance Liquid Chromatography Mass Spectrometry
  • Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry
Agilent 6520 Q-TOF (ESI-HRMS & APCI-HRMS)
  • Non volatile, polar molecules
  • Mass range 100-3000 Da
  • Provide [M+H]+ ion or adducts ions
Waters UPLC-TQD Mass spectrometer (ESI-MS, APCI-MS and LC-MS/MS)
  • Non volatile, polar molecules (ESI) or moderate polar molecules (APCI)
  • Mass range 100-2000 Da
  • Provide [M+H]+ ion or adduct ions