CSIR-Central Drug Research Institute, Lucknow
Supported by: Department of Science & Technology ( Govt. of India)
Mass spectrometry is a powerful analytical technique that is used to determine the molecular weight and calculate molecular formula using high resolution mass spectrometry. The MS principle consists of ionizing chemical compounds to generate charged molecules or molecule fragments and measurement of their mass-to-charge ratios. The combination of liquid chromatography (LC), photodiode-array UV-Vis detector and atmospheric pressure ionization mass spectrometry (APIMS) has proven to be a useful on-line system for establishing the identity of components in given test samples. In recent era, the tandem mass spectrometry (MS/MS) is used to identify unknown compounds, to quantify known compounds, and to elucidate the structure and chemical properties of molecules.