User Manual
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SAIF
Sophisticated Analytical Instrument Facility

CSIR-Central Drug Research Institute, Lucknow

Supported by: Department of Science & Technology ( Govt. of India)

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SAIF Users are requested to register at https://www.saiflucknow.org to enter the information of sample to be submitted and make online payment of sample analysis charges.

Mass spectrometry

Home Mass spectrometry

Mass spectrometry is a powerful analytical technique that is used to determine the molecular weight and calculate the molecular formula using high-resolution mass spectrometry. The MS principle consists of ionizing chemical compounds to generate charged molecules or molecule fragments and measuring their mass-to-charge ratios. The combination of liquid chromatography (LC), photodiode-array UV-Vis detector, and atmospheric pressure ionization mass spectrometry (APIMS) has proven to be a useful on-line system for establishing the identity of components in given test samples. In the recent era, tandem mass spectrometry (MS/MS) is used to identify unknown compounds, to quantify known compounds, and to elucidate the structure and chemical properties of molecules.

SAIF-CDRI LUCKNOW HAS FOLLOWING FACILITIES
  • Electrospray Ionization(ESI)-Mass Spectrometry
  • Desorption Electrospray Ionization (DESI) Mass Spectrometry
  • Atmospheric Pressure Chemical Ionization(APCI)-Mass Spectrometry
  • High-Performance Liquid Chromatography-Mass Spectrometry(LC-MS)
  • High-Performance Liquid Chromatography-Tandem Mass Spectrometry(LC-MS/MS)
  • Electrospray Ionization-High Resolution Mass Spectrometry (ESI-HRMS)
  • Ultra-high Performance Liquid Chromatography- High Resolution Mass Spectrometry(LC-HRMS)
  • Ultra-high Performance Liquid Chromatography- High Resolution Tandem Mass Spectrometry(LC-HRMS/MS)
  • Ion mobility spectrometry(IMS)
  • DESI Mass Spectrometry-Imaging
  • A library of the MS/MS spectrum(https://tmsdatabase.cdri.res.in)

WATERS ALLIANCE E2695/HPLC-TQD MASS SPECTROMETER (ESI/APCI-MS & LC-MS/MS)

  • Non-volatile, polar molecules (ESI) or moderate polar molecules (APCI)
  • Mass range 100-2000 Da
  • Provide [M+H]+ ion or adduct ions
  • MS/MS fragments

NMR

WATERS SYNAPT XS ION MOBILITY TIME-OF-FLIGHT MASS SPECTROMETER (ESI/APCI-HRMS & LC-HRMS/MS)

  • Non-volatile, polar molecules (ESI) or moderate polar molecules (APCI)
  • Mass range 100-3000 Da
  • Provide [M+H]+ ion with accurate mass measurement or adduct ions
  • MS/MS fragments with accurate mass measurement
  • Ion mobility spectrometry (IMS)
  • DESI Mass Spectrometry-Imaging

NMR